Bacterial Growth Requirements Evelyn Lyle ITT Technical Institute Angela Ask‚ MPS January 15‚ 2012 Every organism must find in its environment all of the substances required for energy generation and cellular biosynthesis. The chemicals and elements of this environment that are utilized for bacterial growth are referred to as nutrients. Many bacteria can be identified in the environment by inspection or using genetic techniques. The nutritional requirements of a bacterium such as E Coli are
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calculated the growth curve and it was 1.2. Using this I found the doubling time which was 34s. Introduction Bacteria grows by binary fission. One bacteria becomes two‚ two bacteria becomes four bacteria and it so goes on. A lot of changes occur in the bacterial cell during growth and due to this reason it is difficult to measure growth quantitatively. Growth is therefore usually measure by changing one or two easily measure parameters which will ignore the complicated cellular change. Therefore different
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Chliamydia trachomotisDirect contact (fomites‚ flies) Conjunctivitis #1 cause of blindness in world Bacterial Meningitis Haemophilus influenza type B Direct Contact Endogenous infection: aerosols Fever‚ headache‚ stiff neck‚ nausea‚ vomiting Rare in U.S. Bacterial Meningococcal Meningitis Neisseria meningitidisDirect Contact Droplet Transmission (Aerosols) Petechial rash Global problem Bacterial Pneumococcal Meningitis Streptococcus PneumoniaeDirect Contact Droplet Transmission Aerosols
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Unit 3. Assignment 1: Bacterial Growth Rates 1. Mediums that could be used to determine shigellosis include Btilliant Green Agar‚ and Triple Sugar-Iron Agar. Expected results in a confirmed case of shigellosis are as follows: Brilliant Green Agar – Isolated Shigella colonies which do not ferment lactose or sucrose and appear red or white in color with no growth to trace growth on the Agar plate will be present. Triple Sugar-Iron Agar – Presence of Shigella will manifest as a red slant with
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pelvic and bimanual exam was performed. Swabs of the vaginal area were collected and placed under the microscope. The results were concurrent with the suspected diagnosis of bacterial vaginosis. The physician explained the results of the swab samples and offered the patient different treatment options. Pathophysiology Bacterial Vaginosis (BV) is common infection of the vagina that causes discharge and odor due to the changing environment. The vaginal discharge is “characteristically described as a
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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into three different analysis; microscopy‚ soil microbiology and bacterial growth. The main aim of laboratory work with Escherichia coli and soil sample was to introduce students to bacterial growth in pure culture and soil microbial flora. The experiment of bacterial growth in pure culture using optical density measurement‚ plate count and DAPI direct count‚ showed that Plate Spread method was more precise to understand bacterial population growth tendency and counting generation time. During
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of my microbiology class at Brunswick Community College. This journey started out with a media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with me on the journey to the unknown. As I recall from earlier that
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Fighting Bacterial Growth The purpose of this lab was to determine the effectiveness of antiseptics‚ disinfectants‚ and antibiotics on bacteria. The hypothesis was that if bleach was used‚ it would be the most effective because bleach is commonly used to clean and disinfect various things. The variables that were tested were antibacterial soap and Scope mouthwash for the antiseptics; bleach and ammonia for the disinfectant; and Cipro‚ erythromycin‚ and tetracycline for the antibiotics. All of these
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