outcomes of spontaneous bacterial peritonitis in Egypt‚ single center experience prospective study Spontaneous bacterial peritonitis (SBP) is the infection of the ascetic fluid that occur in the absence of a visceral perforation and in the absence of an intraabdominal inflammatory focus such as abscess‚ acute pancreatitis or cholecystitis (Guarner C and Soriano G‚ 2007). Since its initial description in 1964‚ research has transformed spontaneous bacterial peritonitis (SBP) from a
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LABORATORY EXERCISE 8-A: Preparation of Bacterial Smear and Simple Staining I. INTRODUCTION Bacterial smears are prepared for the purpose of viewing microorganisms under the microscope. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. A bacterial smear is a dried preparation of bacterial cells on a glass slide. Smears may be made from a dry culture
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Chapter 6 Microbial Growth 1 Growth • increase in cellular constituents that may result in: – increase in cell number • e.g.‚ when microorganisms reproduce by budding or binary fission – increase in cell size • e.g.‚ coenocytic microorganisms have nuclear divisions that are not accompanied by cell divisions • microbiologists usually study population growth rather than growth of individual cells 2 The Growth Curve • observed when microorganisms are cultivated in batch
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Bacterial growth patterns and the effects of environmental factors on properties of colonies ERIC LI Abstract In this experiment we work with different types of bacteria and experiment with their different properties and how they grow in certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The
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and record the number of viable cells after a 24-hour incubation period. The measurement of absorbance may be used to analyze bacterial growth since the absorbance or the measure of the turbidity is directly proportional to the number of cells. Turbidity results from the deflection of light by bacterial cells present‚ thus‚ the more turbid the broth is‚ the more bacterial cells are present and the higher the absorbance reading is. There are four characteristic phases of growth of typical
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Practical 9: Examination of Bacterial Plates Objective: 1. To examine bacteria pigmentation‚ colony‚ margin characteristics‚ elevation properties‚ broth characteristics and agar stroke properties. 2. To examine bacteria growth characteristics on different culture media. Introduction: Bacterial species can sometimes be identified on the basis of how they appear on or in the different media. The pigmentation‚ size and shape of bacterial colonies as they grow on and in agar plates can
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Ensuring the Social‚ Political‚ and Technological Protection of Water Quality Protecting Against Bacterial‚ Parasitic‚ and Protozoan Pathogens Nathan Pankowsky Contents Abstract....................................................................................................................................................... 2 Introduction ............................................................................................................................................... 3
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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Amber McMillian May 26‚ 2009 Bacterial Vaginosis This is a vagina condition also referred to as Gardnerella Vaginitis that can produce vaginal discharge that is a result from an overgrowth of normal bacteria. This will cause a woman to have a vaginal discharge with a foul odor. It is not dangerous but it can cause bad symptoms. If any woman has this problem they should also be checked for more serious infections such as Chlamydia and Gonorrhea. The normal symptoms are excessive vagina discharge
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such as Vibrio natriegens‚ who are curved-rod shaped microorganisms‚ they elongate to almost twice their own size and form a dividing wall in which splits the single cell into two daughter cells (Madigan et al.‚ 2015). There are four phases to bacterial cell growth: the lag phase‚ which is a period where bacteria are maturing and adjusting to the environment. During this phase‚ RNA synthesis‚ production of ribosomes‚ enzymes and other molecules occur (Mullenger‚ 1973). This is followed by the exponential
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