Abstract
Abstract
During these laboratories, we observed the cells of diverse types for their general structures. Also, where feasible, observations will be made on subcellular structures. We observed a wide range of cell types representing all Kingdoms. Additionally, these exercises gave us practice using the microscope and preparing specimens for observations.
Introduction
In this lab we learned to observe and recognize all the various types of eukaryotic cells presented in the laboratory. We also learned to observe and recognize the levels of organization of eukaryotes; namely protoplasm, cytoplasm, and nucleoplasm. We learned to differentiate between protist, plant, animal, and fungus cell types. We identified various cell structures set in bold type in the lab instructions. Lastly we compared the relative sixes of cells by estimating the percent of the field occupied. For prokaryotes we observed prepared slides of bacteria under high power of the microscope.
Materials and Methods
Part 1: Eukaryotes
Onion Bulb Epidermis (Plant Kingdom)
During this section the materials were the onion bulb, microscope, water, iodine stain, slide, and cover slip. First we prepared a wet mount of the onion bulb epidermis. We had to pull off a tiny piece and complete the wet mount by using water as a medium. Then we examined the specimen under low power and recorded observations. After reviewing it we added a drop of iodine stain to see how the observations would change. Then examine under high power and label any major structures: Cell wall, Cell membrane, Protoplasm, cytoplasm, nucleoplasm, vacuole, nucleus, nuclear membrane, nucleoli, and the chromatin.
Part 2: Eukaryotes
Elodea (Plant Kingdom)
The materials used were: Elodea, microscope, water, cover slip, and slides. We completed a wet mount by using one leaf of the Elodea plant and water as the medium. WE then observed under high power and labeled the major structures: cells, cell wall, cell...
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