Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
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Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar
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------------------------------------------------- The "Least Count" of any measuring equipment is the smallest quantity that can be measured accurately using that instrument.Thus Least Count indicates the degree of accuracy of measurement that can be achieved by the measuring instrument. All measuring instruments used in physics have a least count. A meter ruler’s least count is 0.1 centimeter; an electronic scale has a least count of 0.001g‚ although this may vary; a vernier caliper has a least count of 0.02 millimeters‚ although
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Livja Koka Anaerobic Bacteria In this experiment the purpose is to seek understanding between an organism and its use of oxygen. Oxygen is a very important part of the organisms’ survival. Oxygen has a tendency to form very reactive by-products inside a cell. These by-products create havoc by reacting with protein and DNA‚ thus inactivating them. Cells that are able to live in the presence of oxygen have evolved enzymes to cope with H2O2 and O2- and thus are not inhibited by O2. Also many anaerobes
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EXECUTIVE SUMMARY … … … … … … … … … … … … … … … … … … … … … … … … … … (Page 3 - 4) ISSUE IDENTIFICATION … … … … … … … … … … … … … … … … … … … … … … … … … … (Page 4) ENVIRONMENTAL & ROOT CAUSE ANALYSIS … … … … … … … … … … … … … … … …. (Page 5 – 8) RECOMMENDATION (S) AND IMPLEMENTATION… … … … … … … … … … … … … … … (Page 9) MONITOR & CONTROL … … … … … … … … … … … … … … … … … … … … … … … … … … (Page 10) CONCLUSION … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … (Page 10) REFERENCES
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The Best Gift I Gave and Received Everyone has their own personal definition of a gift. Some may say a gift is nothing more but an item given from one person to another on a special occasion. From my personal experience I can certainly say that a gift is much more than just an item. A gift brings joy and happiness to me. Receiving a gift always brightens my day. I not only appreciate receiving gifts but I also enjoy giving gifts to both loved ones and less fortunate people‚ who do not receive gifts
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The Count of Monte Cristo: One love‚ a betrayal‚ one revenge Alexandre Dumas‚ one of the most widely read French authors in the world‚ wrote during the late 19th century a prominent romantic historical novel; love‚ betrayal‚ revenge‚ and redemption convert the story of The Count Of Monte- Cristo in one of the most renowned classics of all time. It is considered an extraordinary novel because of its shocking characters‚ theme‚ and drive to vengeance. Alexandre Dumas was born on July 1802 in
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Effectiveness of Garlic in Fighting Bacteria Introduction This experiment determined whether garlic was effective in fighting bacteria. This project interested me because garlic is traditionally used in medicine and is known to combat bacteria. Hypothesis If bacteria is exposed to garlic‚ then the garlic will to abolish the Escherichia coli‚ because the Escherichia coli (E. coli) is usually a weak bacteria. Procedure Have three petri dishes prepared with blood agar‚ and three test tubes
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Count on Me – Bruno Mars If you ever find yourself stuck in the middle of the sea I’ll sail the world to find you If you ever find yourself lost in the dark and you can’t see I’ll be the light to guide you Find out what we’re made of What we are called to help our friends in need You can count on me like one‚ two‚ three I’ll be there and I know when I need it I can count on you like four‚ three‚ two And you’ll be there ’cause that’s what friends Are supposed to do‚ oh yeah‚ ooh‚ ooh If
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INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria‚ yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method
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