The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli), Enterobacter aerogenes (E.aerogenes), Klebsiella pneumoniae (K.pneumoniae), Proteus mirabilis(P.miranilis), Pseudomonas aeruginosa(P.aeruginosa), and Salmonella typhimurium (S.typhimurium). The biochemical tests utilized were; Triple Sugar Iron Agar (TSIA) test, Sulfur Indole Motility (SIM) test, Methyl red test and Voges-Proskauer (MR-VP) test, Citrate test, Urea test,…
The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and, through a series of metabolic tests, identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45, henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment.…
Ehrlich searched for a/an ________. This is a chemical that would hunt down and destroy a pathogen without harming the infected host.…
In the unknown identification labs, we have identified our unknown as Pseudomonas aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant, the P. aeruginosa colonies had a filiform appearance on the edges.…
Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had, was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane, periplasmic space, peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any colony pigment. It appeared as a circular, raised, undulated bacterium. The arrangement of Pseudomonas aeruginosa is rod shaped. Multiple biochemical tests can be performed to determine if gram positive or negative. See Table below:…
The plate count agar, CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly, the plate count agar is a medium for the enumeration of viable organisms in food, water, waste water and also from clinical samples, and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas species. If the Pseudomonas species are present, a blue-green or brown pigmentation under the daylight, or fluorescence can be taken as a presumptive evidence under UV light. In the experiment, we had observed the colonies under UV light and…
Gram staining showed gram negative rods, a motility test was also conducted to see if the bacterium moved or not, it was found to be none motile. Three different types of agar plates were used, they had two known bacterium put on along with the unknown to be able to compare negative and positive results if the known with the results of the unknown, refer to Barbaro (2016) for how the test were done.…
The purpose of this experiment was to find the identity two unknown bacteria in the given test tube.…
My unkown teste to be variable to both bacitracin and enterococcosel test. Bacitacin test was carried out to determine if bacitracin antibiotic would prevent the synthesis of peptidoglycan on my unknown organism cell wall, sensitivity of bacitrin would be seen by the formation any zone of inhibition around the bacitracin disk meaning growth was not possible. My unknown was variable to bacitracin because there was growth on one side of the disk with the other side showing no growth at…
The unknown microorganism #5 is Pseudomonas aeruginosa. It can survive in a variety of environments and at temperatures found in clinical settings (Murray et al, 1999). Clinical setting is referring to temperatures such as those in the hospital environment. P. aeruginosa can also survive in aqueous environments. Pseudomonas aeruginosa is found at several different sources. Nosocomial sources of this microorganism are: respiratory therapy equipment, eye drops, dialysis fluid, and disinfectants. It can also be found in the GI tract and moist areas on the human body. Some other sources of Pseudomonas aeruginosa are: contact lens solution, cosmetics, and pools. However it is not commonly found as the normal flora on the human…
Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species, one can look at the metabolic differences (fermentation), as well as the environmental condition differences (temperature, pH, oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media can be used to culture and identify bacteria.…
This lab focuses on the isolation and characterization of an unknown organisms expressing interesting properties in relation to Antimicrobial Activity. At the conclusion of this laboratory experience The researcher would be required to describe the isolates obtained from the soil macroscopic and microscopic appearance, Perform the gram stain of the microorganisms in question and to discriminate the organisms from other microbes that could be contained in the soil and to finally make certain that the organisms have not been exposed to outside sources and be isolated in a pure culture. The researcher must first isolate the microbe and try to grow the organism in a pure culture to commence with testing the organism. The isolation of Microbes in a laboratory and clinical settings are of the up most importance and due to the isolation of different microbes from each other various procedures become open for the…
Primary isolation was initiated by nutrient agar plating and subsequently transferred to selective media like Pseudomonas Isolation Agar, MacConkey Agar and EMB agar for better isolation. Using sterile inoculation loop colonies of the test organism are transferred to 5ml of sterile nutrient broth and incubated at 37 0C overnight for 24hrs. Then this bacterial culture were suspended in saline solution (0.85%Nacl) and adjusted to a turbidity of 0.5 Mac Farland standards (108cfu/ml). This suspension was used for preliminary screening of anti bacterial…
13. there was no inhibition of bacterial growth around the P disk. This is indicative of viridans streptococci. this is a collection of streptococci normally found in the oral cavity.…
Bacteria are among the oldest species in the world.They both incerease in number and comply with nature very quikly so,they maintain their species.Therefore,bacteria have been the most common type of species according to number.Hence,bacteria had been classified in order to make analysis.easily.Bacteria can be classified into three main categories on the basis of their nurition:saprophytic,parasitic and chemoautotrophic bacteria.…